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iFluor® 350 Anti-human CD22 Antibody
HIB22
HIB22 is an anti-human monoclonal antibody that recognizes the CD22 antigen. CD22 (also known as BL-CAM or Lyb8) is a 120 - 130 kD glycoprotein that is located on the surface of cells like B cells and stem cells. CD22 plays a role in essential cellular pathways, namely, the negative regulation of B cell receptor signaling pathway. Moreover, in many organisms, it is a negative regulator of calcium-mediated signaling, suppresses immunoglobulin secretion and is involved in the negative regulation of B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like PI3-kinase, PLCg1, p53/56lyn and CD45. CD22 is a fairly uncommon antibody target, with a little more than 4000 publications in the last decade. Even still, CD22 is vital to cell biology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 350 (ex/em = 345/450 nm). It is compatible with the 355 nm laser and 447/60 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10220010 | 100 tests | Price | |
| 10220011 | 500 tests | Price |
Physical properties
| Molecular weight | N/A |
| Solvent | water |
Antibody properties
| Other names | BL-CAM, Siglec-2 |
| Clone | HIB22 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.83 |
| Correction factor (280 nm) | 0.23 |
| Extinction coefficient (cm -1 M -1) | 20000 1 |
| Excitation (nm) | 345 |
| Emission (nm) | 450 |
| Quantum yield | 0.95 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on April 26, 2026