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About
iFluor® 405 Anti-human CD47 Antibody
HIRH47
HIRH47 is an anti-human monoclonal antibody that targets the CD47 antigen. CD47 (sometimes referred to as MER6, Integrin associated protein or Rh-associated protein) is a 42 - 52 kD multi-pass membrane protein that is found on the surface of cells like T cells. In some organisms, CD47 upregulates cell population proliferation, acts to positively regulate phagocytosis and is involved in the positive regulation of stress fiber assembly. In addition, it is a component of key cellular pathways, namely, the integrin-mediated signaling pathway and negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like SIRP, Thrombospondin and CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 405 (ex/em = 403/427 nm). It is compatible with the 405 nm laser and 440/50 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10470020 | 100 tests | Price | |
| 10470021 | 500 tests | Price |
Antibody properties
| Other names | gp42; IAP; neurophilin; MER6; Integrin associated protein |
| Clone | HIRH47 |
| Host | mouse |
| Isotype | mouse IgG1 |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.48 |
| Correction factor (280 nm) | 0.77 |
| Extinction coefficient (cm -1 M -1) | 37000 1 |
| Excitation (nm) | 403 |
| Emission (nm) | 427 |
| Quantum yield | 0.91 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 18, 2025