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iFluor® 405 Anti-human CD53 Antibody
HI29
HI29 is an anti-human monoclonal antibody that targets the CD53 antigen. CD53 (sometimes referred to as Tetraspanin-25, MOX44 or OX44) is a 35 - 42 kD member of the tetraspan family that is found on the surface of cells such as NK cells. In some organisms, CD53 enhances myoblast fusion, and is associated with a variety of biologically interesting macromolecules/ligands, in particular, VLA-4, Integrins and HLA-DR. CD53 is a relatively rare antibody target, with fewer than 600 publications in the last decade. Even still, CD53 has a variety of applications in costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 405 (ex/em = 403/427 nm). It is compatible with the 405 nm laser and 420/10 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10530020 | 100 tests | Price | |
| 10530021 | 500 tests | Price |
Antibody properties
| Other names | Tetraspanin-25, MOX44 |
| Clone | HI29 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.48 |
| Correction factor (280 nm) | 0.77 |
| Extinction coefficient (cm -1 M -1) | 37000 1 |
| Excitation (nm) | 403 |
| Emission (nm) | 427 |
| Quantum yield | 0.91 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 13, 2026