Name: iFluor® 405 Anti-human CD54 Antibody *R6-5-D6*
Brand: AAT Bioquest
SKU: 10540020
Availability: InStock

iFluor® 405 Anti-human CD54 Antibody

R6-5-D6

R6-5-D6 is an anti-human monoclonal antibody that targets the CD54 antigen. CD54 (also known as ICAM-1, ICAM1 or Ly-47) is a 85 - 110 kD member of the Ig superfamily that is expressed on the surface of cells such as B cells, endothelial cells and macrophages. CD54 is associated with a variety of biologically interesting macromolecules/ligands, for example, LFA-1, Mac-1 and Rhinovirus. CD54 is a fairly uncommon antibody target, with a little more than 4400 publications in the last decade. Even still, CD54 is essential for costimulatory molecules, neuroscience and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 405 (ex/em = 403/427 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the Beckman Coulter Navios EX).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10540020	100 tests	Price
10540021	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd54 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	ICAM-1
Clone	R6-5-D6
Host	mouse
Isotype	mouse igg2a
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.48
Correction factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000 ¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 29, 2025