Products
Technologies
Applications
Services
Resources
Selection Guides
About
iFluor® 405 Anti-human CD8 Antibody
HIT8a
The HIT8a monoclonal antibody binds to human CD8a, a 32 - 34 kD member of the Ig superfamily often located on the surface of dendritic cells, t cells and thymocytes. CD8 is a component of important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 405 (ex/em = 403/427 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10080020 | 100 tests | Price | |
| 10080021 | 500 tests | Price |
Antibody properties
| Other names | T8; Leu2 |
| Clone | HIT8a |
| Host | mouse |
| Isotype | mouse IgG1 |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.48 |
| Correction factor (280 nm) | 0.77 |
| Extinction coefficient (cm -1 M -1) | 37000 1 |
| Excitation (nm) | 403 |
| Emission (nm) | 427 |
| Quantum yield | 0.91 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 22, 2025