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iFluor® 405 goat anti-mouse IgG (H+L)
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 405 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~401 nm and ~420 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 405 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 405 goat anti-mouse IgG (H&amp;L). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the Pacific Blue channel (Ex/Em=405/445 nm).&nbsp;</p>
<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 405 goat anti-mouse IgG (H&amp;L). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the Pacific Blue channel (Ex/Em=405/445 nm).&nbsp;</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16444200 ug
Price
167311 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Correction factor (260 nm)0.48
Correction factor (280 nm)0.77
Extinction coefficient (cm -1 M -1)
37000
1
Excitation (nm)403
Emission (nm)427
Quantum yield
0.91
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025