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iFluor® 430 Anti-human CD7 Antibody *HIT7*

Ordering information
Price ()
Catalog Number10070030
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
Solventwater
Spectral properties
Correction Factor (260 nm)0.68
Correction Factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)433
Emission (nm)498
Quantum yield0.781
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Other names
gp40, TP41
Clone
HIT7
Host
mouse
Isotype
mouse IgG1
Reactivity
human
Application
Flow Cytometry (FACS), Fluorescence Imaging
Molecular weight
N/A
Correction Factor (260 nm)
0.68
Correction Factor (280 nm)
0.3
Extinction coefficient (cm -1 M -1)
400001
Excitation (nm)
433
Emission (nm)
498
Quantum yield
0.781
HIT7 is an anti-human monoclonal antibody that recognizes the CD7 antigen. CD7 (also known as gp40) is a 40 kD single-pass type I membrane protein that is located on the surface of cells like NK cells, stem cells and T cells. CD7 has been closely linked to essential biological processes such as immune response, particularly adaptive immune response. Furthermore, it is involved with important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like PI3-Kinase. CD7 is a fairly uncommon antibody target, with a little more than 3600 publications in the last decade. Even still, CD7 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to iFluor® 430 (ex/em = 433/498 nm). It is compatible with the 445 nm laser and 510/80 nm bandpass filter (for example, as in the BD FACSAria™ III).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.68
Correction Factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)433
Emission (nm)498
Quantum yield0.781

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of iFluor® 430 Anti-human CD7 Antibody *HIT7* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.