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AAT Bioquest

iFluor® 430 Anti-human CD95 Antibody *EOS9.1*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.68
Correction Factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)433
Emission (nm)498
Quantum yield0.781
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
Fas; TNFRSF6; APO-1; FASLG
Clone
EOS9.1
Host
mouse
Isotype
Mouse IgM kappa
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.68
Correction Factor (280 nm)
0.3
Extinction coefficient (cm -1 M -1)
400001
Excitation (nm)
433
Emission (nm)
498
Quantum yield
0.781
EOS9.1 is an anti-human monoclonal antibody that forms an immune complex with the CD95 antigen. CD95 (alternatively called APO-1 or TNFRSF6) is a 45 kD member of the TNFR superfamily that is found on the surface of cells like NK cells, T cells and granulocytes. CD95 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD178 (fas ligand). CD95 is a moderately popular antibody target, with over 12000 publications in the last decade. CD95 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of cell biology. This antibody was purified through affinity chromatography and conjugated to iFluor® 430 (ex/em = 433/498 nm). It is compatible with the 445 nm laser and 510/80 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.68
Correction Factor (280 nm)0.3
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)433
Emission (nm)498
Quantum yield0.781

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd95 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.