Name: iFluor® 450 Anti-human CD1 Antibody *HI149*
Brand: AAT Bioquest
SKU: 10010040
Availability: InStock

iFluor® 450 Anti-human CD1 Antibody

HI149

HI149 is an anti-human monoclonal antibody that forms an immune complex with the CD1a antigen. CD1a (sometimes called R4 or T6) is a 49 kD member of the Ig superfamily that is found on the surface of cells like macrophages, dendritic cells and T cells. In certain organisms, CD1 is involved in the positive regulation of T cell mediated cytotoxicity, and is associated with a variety of biologically interesting macromolecules/ligands, namely, β-2-Microglobulin. CD1 is a moderately popular antibody target, with over 15000 publications in the last decade. CD1a is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 450 (ex/em = 451/502 nm). It is compatible with the 445 nm laser and 510/80 nm bandpass filter (for example, as in the BD FACSAria™ III).

Protocol

COA

Catalog	Size	Price	Quantity
10010040	100 tests	Price
10010041	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd1a antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	R4; T6
Clone	HI149
Host	mouse
Isotype	mouse IgG1
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.45
Correction factor (280 nm)	0.27
Extinction coefficient (cm ^-1 M ^-1)	40000 ¹
Excitation (nm)	451
Emission (nm)	502
Quantum yield	0.82 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025