Name: iFluor® 488 Anti-human CD148 Antibody *MEM-CD148/05*
Brand: AAT Bioquest
SKU: 11480050
Availability: InStock

iFluor® 488 Anti-human CD148 Antibody

MEM-CD148/05

MEM-CD148/05 is an anti-human monoclonal antibody that forms an immune complex with the CD148 antigen. CD148 (sometimes called p260 or DEP-1) is a glycoprotein that is found on the surface of cells such as dendritic cells, platelets, B cells and NK cells. CD148 acts in key cellular pathways, for instance, the T cell receptor signaling pathway, negative regulation of epidermal growth factor receptor signaling pathway and positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis. In addition, in many organisms, it upregulates MAPK cascade, is an inhibitor of cell growth and represses cell migration. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands. CD148 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD148 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSAria™ II).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11480050	100 tests	Price
11480051	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd148 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	DEP-1, p260
Clone	MEM-CD148/05
Host	Mouse
Isotype	Mouse IgG2b
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 15, 2025