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iFluor® 488 Anti-human CD158d Antibody *mAb#33*

The mAb#33 monoclonal antibody binds to human CD158d, a 45 - 50 kD transmembrane glycoprotein frequently expressed on the surface of natural killer cells. In certain organisms, CD158d negatively regulates natural killer cell mediated cytotoxicity, is an enhancer of cellular senescence and positively regulates natural killer cell cytokine production. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like HLA-Bw4. CD158d is a relatively rare antibody target, with fewer than 200 publications in the last decade. Even still, CD158d has been widely used in immunology and innate immunity research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD LSRFortessa™ X-20).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of cd158d antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 31, 2024

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Antibody properties

Other namesKIR2DL4
ClonemAb#33
Hostmouse
IsotypeMouse IgG1 kappa
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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