Name: iFluor® 488 Anti-human CD2 Antibody *HIT11*
Brand: AAT Bioquest
SKU: 10020050
Availability: InStock

iFluor® 488 Anti-human CD2 Antibody

HIT11 is an anti-human monoclonal antibody that recognizes the CD2 antigen. CD2 (sometimes referred to as LFA-2) is a 45 kD single-pass type I membrane protein that is found on the surface of cells like T cells. CD2 has been thought to be involved with key biological processes such as cell-cell adhesion, especially heterotypic cell-cell adhesion. Also, in some organisms, it enhances myeloid dendritic cell activation, acts to positively regulate interferon-gamma secretion and is an enhancer of tumor necrosis factor production. CD2 is involved with key cellular pathways, for instance, the cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with critical macromolecules/ligands such as LFA-3, CD59, CD58 and CD48. CD2 is a moderately popular antibody target, with over 16000 publications in the last decade. CD2 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSAria™ II).

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 Anti-human CD2 Antibody *HIT11*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B2-A channel.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
10020050	100 tests	Price
10020051	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD2 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	LFA-2, Erythrocyte R, T11
Clone	HIT11
Host	Mouse
Isotype	IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 19, 2026