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iFluor® 488 Anti-human CD3 Antibody
HIT3a
HIT3a is an anti-human monoclonal antibody that recognizes the CD3e antigen. CD3e (sometimes referred to as T cell antigen receptor complex or T3E) is a 20 kD member of the Ig superfamily that is found on the surface of cells like T cells. CD3 acts in essential cellular pathways, namely, the G protein-coupled receptor signaling pathway, apoptotic signaling pathway and T cell receptor signaling pathway. In addition, in certain organisms, it positively regulates T cell anergy, acts to positively regulate cell-matrix adhesion and is involved in the positive regulation of calcium-mediated signaling. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSCanto™ II).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 Anti-human CD3 Antibody *HIT3a*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B2-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 Anti-human CD3 Antibody *HIT3a*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B2-A channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
10030050100 tests
Price
10030051500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT3E
CloneHIT3a
Hostmouse
Isotypemouse IgG2a
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 1, 2025