Name: iFluor® 488 Anti-human CD3 Antibody *SK7*
Brand: AAT Bioquest
SKU: 10033050
Availability: InStock

iFluor® 488 Anti-human CD3 Antibody

SK7

The SK7 monoclonal antibody reacts with human CD3e, a 20 kD member of the Ig superfamily often found on the surface of t cells, tregs and nkt cells. In many organisms, CD3 positively regulates interleukin-2 biosynthetic process, enhances cell-matrix adhesion and is a positive regulator of gene expression. Moreover, it plays a role in essential cellular pathways, for example, the T cell receptor signaling pathway, negative regulation of smoothened signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 525/50 nm bandpass filter (for example, as in the Miltenyi Biotec MACSQuant Analyzer 10).

Flow cytometry analysis of whole blood cells stained with iFluor® 488 anti-human CD3 antibody (Clone: SK7). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the iFluor® 488 specific B2-A channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10033050	100 tests	Price
10033051	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	T3E
Clone	SK7
Host	mouse
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025