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iFluor® 488 Anti-human CD45 Antibody
HI185
The HI185 monoclonal antibody reacts with human CD45, a 180 - 240 kD transmembrane glycoprotein often found on the surface of neutrophils, hematopoietic cells, B cells and dendritic cells. CD45 is a component of vital cellular pathways, for example, the T cell receptor signaling pathway, negative regulation of cytokine-mediated signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. Also, in many organisms, it enhances hematopoietic stem cell migration, is a suppressor of cytokine-mediated signaling pathway and enhances protein tyrosine phosphatase activity. CD45 has been thought to be involved with vital biological processes such as dephosphorylation, especially protein dephosphorylation, and is associated with a variety of biologically interesting macromolecules/ligands, for example, p59fyn and Src kinases. CD45 is a very popular antibody target, with over 50000 publications in the last decade. CD45 is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of neuroscience. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 530/30 nm bandpass filter (for example, as in the BD FACSAria™ III).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 10453050 | 100 tests | Price | |
| 10453051 | 500 tests | Price |
Antibody properties
| Other names | Leukocyte Common Antigen (LCA); T200; PTPRC |
| Clone | HI185 |
| Host | mouse |
| Isotype | mouse IgG1 |
| Reactivity | human |
| Application | Flow Cytometry (FACS); Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.21 |
| Correction factor (280 nm) | 0.11 |
| Extinction coefficient (cm -1 M -1) | 75000 1 |
| Excitation (nm) | 491 |
| Emission (nm) | 516 |
| Quantum yield | 0.9 1 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on November 3, 2025