logo
AAT Bioquest

iFluor® 488 Anti-human CD8 Antibody *SK1*

Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weightN/A
Solventwater
Spectral properties
Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)491
Emission (nm)516
Quantum yield0.91
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Other names
T8; Leu2
Clone
SK1
Host
mouse
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Molecular weight
N/A
Correction Factor (260 nm)
0.21
Correction Factor (280 nm)
0.11
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
491
Emission (nm)
516
Quantum yield
0.91
SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. CD8a (sometimes called MAL or Leu2) is a 32 - 34 kD member of the Ig superfamily that is expressed on the surface of cells such as NK cells and T cells. CD8 is a component of important cellular pathways, for example, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 515/20 nm bandpass filter (for example, as in the BD FACSymphony™ A5).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)491
Emission (nm)516
Quantum yield0.91

Antibody Finder


Select Your CD Antibody
Select Your Clone
Select Your Conjugate

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd8a antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.