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iFluor® 488 Anti-human CD8 Antibody
SK1
SK1 is an anti-human monoclonal antibody that is specific for the CD8a antigen. CD8a (sometimes called MAL or Leu2) is a 32 - 34 kD member of the Ig superfamily that is expressed on the surface of cells such as NK cells and T cells. CD8 is a component of important cellular pathways, for example, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 515/20 nm bandpass filter (for example, as in the BD FACSymphony™ A5).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific B2-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 488 Anti-human CD8 Antibody *SK1*. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific B2-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
10081050100 tests
Price
10081051500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT8; Leu2
CloneSK1
Hostmouse
Reactivityhuman
ApplicationFlow Cytometry (FACS); Fluorescence Imaging
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
Documents
Protocol
Safety Data Sheet
Safety Data Sheet
Certificate of Analysis
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Page updated on October 21, 2025