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iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).
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Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)491
Emission (nm)516
Quantum yield0.91
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate
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OverviewpdfSDSpdfProtocol


Molecular weight
~150000
Correction Factor (260 nm)
0.21
Correction Factor (280 nm)
0.11
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
491
Emission (nm)
516
Quantum yield
0.91
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.21
Correction Factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)491
Emission (nm)516
Quantum yield0.91

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L)4034273700010.9110.480.77
iFluor® 514 goat anti-mouse IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L)5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L)55757010000010.6410.230.14
iFluor® 594 goat anti-mouse IgG (H+L)58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L)64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L)65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 488 goat anti-rabbit IgG (H+L)4915167500010.910.210.11
iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 546 goat anti-mouse IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-mouse IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-mouse IgG (H+L) 80182025000010.1110.030.08
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-mouse IgG (H+L) 81182225000010.0510.090.15
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-mouse IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-mouse IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-mouse IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Images


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References


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TTF-1 expression in ovarian and uterine epithelial neoplasia and its potential significance, an immunohistochemical assessment with multiple monoclonal antibodies and different secondary detection systems
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Serum anti-GAGA4 IgM antibodies differentiate relapsing remitting and secondary progressive multiple sclerosis from primary progressive multiple sclerosis and other neurological diseases
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