iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~150000 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
iFluor® 488 goat anti-mouse IgG (H+L) |
Overview | ![]() ![]() |
See also: Anti-Mouse Secondary Antibodies, Confocal Microscopy, iFluor® Secondary Antibodies, iFluor Secondary Reagents, Immunofluorescence, Western Blotting Assays
Molecular weight ~150000 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 goat anti-mouse IgG (H+L) | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 goat anti-mouse IgG (H+L) | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 514 goat anti-mouse IgG (H+L) | 511 | 527 | 750001 | 0.831 | 0.265 | 0.116 |
iFluor® 532 goat anti-mouse IgG (H+L) | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 555 goat anti-mouse IgG (H+L) | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 goat anti-mouse IgG (H+L) | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
iFluor® 633 goat anti-mouse IgG (H+L) | 640 | 654 | 2500001 | 0.291 | 0.062 | 0.044 |
iFluor® 647 goat anti-mouse IgG (H+L) | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 680 goat anti-mouse IgG (H+L) | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
Show More (31) |
Images
Citations
View all 13 citations: Citation Explorer
Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma
Authors: Song, Feifeng and Zhang, Qi and Lu, Xixuan and Xu, Tong and Hu, Qing and Hu, Xiaoping and Fan, Weijiao and Zhang, Yiwen and Huang, Ping
Journal: Journal of Physiology and Biochemistry (2023): 1--14
Authors: Song, Feifeng and Zhang, Qi and Lu, Xixuan and Xu, Tong and Hu, Qing and Hu, Xiaoping and Fan, Weijiao and Zhang, Yiwen and Huang, Ping
Journal: Journal of Physiology and Biochemistry (2023): 1--14
Upregulation of HMGB1 in tumor-associated macrophages induced by tumor cell-derived lactate further promotes colorectal cancer progression
Authors: Gao, Xinyi and Zhou, Shiqi and Qin, Zhaofu and Li, Dechuan and Zhu, Yuping and Ma, Dening
Journal: Journal of Translational Medicine (2023): 1--13
Authors: Gao, Xinyi and Zhou, Shiqi and Qin, Zhaofu and Li, Dechuan and Zhu, Yuping and Ma, Dening
Journal: Journal of Translational Medicine (2023): 1--13
Oxidative stress biomarker triggered multiplexed tool for auxiliary diagnosis of atherosclerosis
Authors: Ma, Yuan and Sun, Wei and Ye, Zhifei and Liu, Liuhui and Li, Menghuan and Shang, Jinhui and Xu, Xinyu and Cao, Hui and Xu, Li and Liu, Yongchao and others,
Journal: Science Advances (2023): eadh1037
Authors: Ma, Yuan and Sun, Wei and Ye, Zhifei and Liu, Liuhui and Li, Menghuan and Shang, Jinhui and Xu, Xinyu and Cao, Hui and Xu, Li and Liu, Yongchao and others,
Journal: Science Advances (2023): eadh1037
Liensinine Inhibits Mitophagy to Potentiate Mitochondria-Targeting Artemisinin-Loaded Polymer Nanostructures for Breast Cancer Therapy
Authors: Li, Jia-Mi and Yu, Hui and Deng, Kai and Wang, Qian and Li, Kun-Heng and Huang, Shi-Wen
Journal: ACS Applied Nano Materials (2023)
Authors: Li, Jia-Mi and Yu, Hui and Deng, Kai and Wang, Qian and Li, Kun-Heng and Huang, Shi-Wen
Journal: ACS Applied Nano Materials (2023)
Enhancement of pacing function by HCN4 overexpression in human pluripotent stem cell-derived cardiomyocytes
Authors: Saito, Yukihiro and Nakamura, Kazufumi and Yoshida, Masashi and Sugiyama, Hiroki and Akagi, Satoshi and Miyoshi, Toru and Morita, Hiroshi and Ito, Hiroshi
Journal: Stem Cell Research \& Therapy (2022): 1--13
Authors: Saito, Yukihiro and Nakamura, Kazufumi and Yoshida, Masashi and Sugiyama, Hiroki and Akagi, Satoshi and Miyoshi, Toru and Morita, Hiroshi and Ito, Hiroshi
Journal: Stem Cell Research \& Therapy (2022): 1--13
The Bioactive Phenolic Agents Diaryl Ether CVB2-61 and Diarylheptanoid CVB4-57 as Connexin Hemichannel Blocker
Authors: Dierks, Anne and Vanucci-Bacqu{\'e}, Corinne and Sch{\"a}fer, Anne-Marie and Lehrich, Tina and Ruhe, Frederike and Schadzek, Patrik and Bedos-Belval, Florence and Ngezahayo, Anaclet
Journal: (2022)
Authors: Dierks, Anne and Vanucci-Bacqu{\'e}, Corinne and Sch{\"a}fer, Anne-Marie and Lehrich, Tina and Ruhe, Frederike and Schadzek, Patrik and Bedos-Belval, Florence and Ngezahayo, Anaclet
Journal: (2022)
Multifunctional Metal--Organic Framework Exoskeletons Protect Biohybrid Sperm Microrobots for Active Drug Delivery from the Surrounding Threats
Authors: Chen, Qiwei and Tang, Songsong and Li, Yangyang and Cong, Zhaoqing and Lu, Dongdong and Yang, Qingxin and Zhang, Xueji and Wu, Song
Journal: ACS Applied Materials \& Interfaces (2021): 58382--58392
Authors: Chen, Qiwei and Tang, Songsong and Li, Yangyang and Cong, Zhaoqing and Lu, Dongdong and Yang, Qingxin and Zhang, Xueji and Wu, Song
Journal: ACS Applied Materials \& Interfaces (2021): 58382--58392
Stimulation of the A
Authors: Connexin26, Receptor Subtype Enhances
Journal: Cell Physiol Biochem (2019): 606--622
Authors: Connexin26, Receptor Subtype Enhances
Journal: Cell Physiol Biochem (2019): 606--622
Phospholipase Cγ2 is critical for Ca 2+ flux and cytokine production in anti-fungal innate immunity of human corneal epithelial cells
Authors: Peng, Xudong and Zhao, Guiqiu and Lin, Jing and Qu, Jianqiu and Zhang, Yingxue and Li, Cui
Journal: BMC ophthalmology (2018): 170
Authors: Peng, Xudong and Zhao, Guiqiu and Lin, Jing and Qu, Jianqiu and Zhang, Yingxue and Li, Cui
Journal: BMC ophthalmology (2018): 170
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
References
View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures
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Parkinsonism/dystonia syndrome secondary to multiple sclerosis with anti-basal-ganglia antibodies
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Neutralizing antibodies and secondary therapy failure after treatment with botulinum toxin type A: much ado about nothing
Authors: Lange O, Bigalke H, Dengler R, Wegner F, deGroot M, Wohlfarth K.
Journal: Clin Neuropharmacol (2009): 213
Authors: Lange O, Bigalke H, Dengler R, Wegner F, deGroot M, Wohlfarth K.
Journal: Clin Neuropharmacol (2009): 213
Application notes
FAQ
I am performing an ELISA using biotinylated goat secondary antibodies, can you recommend a working concentration?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?