Name: iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*
Brand: AAT Bioquest
SKU: 16528
Availability: InStock

iFluor® 488 goat anti-mouse IgG (H+L)

Cross Adsorbed

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor® 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).

Protocol

COA

Catalog	Size	Price	Quantity
16528	200 ug	Price
16773	1 mg	Price

Citations

View all 22 citations: Citation Explorer

Glutamatergic heterogeneity in the neuropeptide projections from the lateral hypothalamus to the mouse olfactory bulb

Authors:

Qi, Meizhu and Won, Julia and Rodriguez, Catherine and Storace, Douglas Anthony

Journal:

bioRxiv (2025): 2025--02

Repression of Connexin26 Hemichannel activity protects the Barrier Function of Respiratory Airway Epithelial Cells against LPS-induced Alteration

Authors:

Lehrich, Tina and Dierks, Anne and Plenge, Masina and Obernolte, Helena and Grieger, Klaudia and Sewald, Katherina and Rodriguez, Frederic and Malet, Lucie and Braubach, Peter and Bedos-Belval, Florence and others,

SLC7A5 regulates tryptophan uptake and PD-L1 expression levels via the kynurenine pathway in ovarian cancer

Authors:

Jiang, Ruibin and Jin, Bo and Sun, Yuting and Chen, Zhongjian and Wan, Danying and Feng, Jianguo and Ying, Lisha and Peng, Chanjuan and Gu, Linhui

Journal:

Oncology Letters (2025): 1--15

NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer

Authors:

Wu, Chengwei and Wang, Song and Huang, Tao and Xi, Xinran and Xu, Lishuai and Wang, Jiawei and Hou, Yinfen and Xia, Yabin and Xu, Li and Wang, Luman and others,

Journal:

Cell Biology and Toxicology (2024): 93

The CD163/TWEAK/Fn14 axis: A potential therapeutic target for alleviating inflammatory bone loss

Authors:

Qian, Ji-kun and Ma, Yuan and Huang, Xuan and Li, Xiao-ran and Xu, Ya-fei and Liu, Zi-ying and Gu, Yuan and Shen, Ke and Tian, Liang-jie and Wang, Yu-tian and others,

Journal:

Journal of Orthopaedic Translation (2024): 82--95

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding

Authors:

Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.

Journal:

Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers

Authors:

Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.

Journal:

Oncotarget (2011): 728

Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient

Authors:

Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.

Journal:

Pharmacotherapy (2010): 954

Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies

Authors:

Azzopardi P, Forrester M, Ehtisham S.

Journal:

J Paediatr Child Health (2010): 439

Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies

Authors:

Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.

Journal:

Anal Chim Acta (2010): 126

Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 16528)
Safety Data Sheet (Catalog 16773)
Certificate of Analysis

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Page updated on October 27, 2025