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iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 488 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~491 nm and ~514 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 488 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 488 goat anti-mouse IgG (H+L)4915167500010.910.210.11
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 350 goat anti-rabbit IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-rabbit IgG (H+L)4034273700010.9110.480.77
iFluor® 514 goat anti-rabbit IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-rabbit IgG (H+L)5375609000010.6810.260.16
iFluor® 555 goat anti-rabbit IgG (H+L)55757010000010.6410.230.14
iFluor® 594 goat anti-rabbit IgG (H+L)58760320000010.5310.050.04
iFluor® 633 goat anti-rabbit IgG (H+L)64065425000010.2910.0620.044
iFluor® 647 goat anti-rabbit IgG (H+L)65667025000010.2510.030.03
iFluor® 680 goat anti-rabbit IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-rabbit IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-rabbit IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-rabbit IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 546 goat anti-rabbit IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-rabbit IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-rabbit IgG (H+L)80182025000010.1110.030.08
iFluor® 800 goat anti-rabbit IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-rabbit IgG (H+L)81182225000010.0510.090.15
iFluor® 810 goat anti-rabbit IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-rabbit IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-rabbit IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-rabbit IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-rabbit IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-rabbit IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-rabbit IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Citations

View all 21 citations: Citation Explorer
NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer
Authors: Wu, Chengwei and Wang, Song and Huang, Tao and Xi, Xinran and Xu, Lishuai and Wang, Jiawei and Hou, Yinfen and Xia, Yabin and Xu, Li and Wang, Luman and others,
Journal: Cell Biology and Toxicology (2024): 93
Extracellular vesicles from alveolar macrophages harboring phagocytosed methicillin-resistant Staphylococcus aureus induce necroptosis
Authors: Bai, Songjie and Wen, Xuehuan and Li, Bingyu and Hu, Ruomeng and Yang, Jie and Yu, Qing and Zeng, Xianchang and Feng, Huajun and Zhu, Feng and Cai, Zhijian and others,
Journal: Cell Reports (2024)
High Intensity Focused Ultrasound-Driven Nanomotor for Effective Ferroptosis-Immunotherapy of TNBC
Authors: Yu, Xiangrong and Li, Xuejing and Chen, Qingwang and Wang, Siyu and Xu, Ruizhe and He, Ying and Qin, Xifeng and Zhang, Jun and Yang, Wuli and Shi, Leming and others,
Journal: Advanced Science (2024): 2305546
Effects of matrix viscoelasticity on cell--matrix interaction, actin cytoskeleton organization, and apoptosis of osteosarcoma MG-63 cells
Authors: Deng, Huan and Wang, Yao and Yin, Yue and Shu, Jun and Zhang, Junwei and Shu, Xuedong and Wu, Fang and He, Jing
Journal: Journal of Materials Chemistry B (2024)

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Page updated on November 9, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
1667816828
Quantity
Add to cart

Additional ordering information

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Physical properties

Molecular weight

~150000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
HeLa cells were stained with rabbit anti-tubulin followed by iFluor 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*, and nuclei were stained Nuclear Blue DCS1 (Cat#17548).
HeLa cells were stained with rabbit anti-tubulin followed by iFluor 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*, and nuclei were stained Nuclear Blue DCS1 (Cat#17548).
HeLa cells were stained with rabbit anti-tubulin followed by iFluor 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*, and nuclei were stained Nuclear Blue DCS1 (Cat#17548).