logo
Products
Services
Resources
Selection Guides
About
iFluor® 514 Anti-human CD19 Antibody
HIB19
HIB19 is an anti-human monoclonal antibody that recognizes the CD19 antigen. CD19 (also known as CVID3) is a 95 kD glycoprotein that is located on the surface of cells like stem cells, dendritic cells and B cells. In certain organisms, CD19 promotes release of sequestered calcium ion into cytosol, plays a role in the upregulation of phosphatidylinositol 3-kinase activity and acts to positively regulate protein kinase B signaling. In addition, it is a member of critical cellular pathways, for example, the antigen receptor-mediated signaling pathway and B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as lyn. CD19 is a very popular antibody target, with over 36000 publications in the last decade. CD19 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 514 Anti-human CD19 Antibody (HIB19). The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B3-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 514 Anti-human CD19 Antibody (HIB19). The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B3-A channel.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
10192060100 tests
Price
10192061500 tests
Price
 
Antibody properties
Other namesB4
CloneHIB19
HostMouse
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Absorbance (nm)511
Correction factor (260 nm)0.265
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)511
Emission (nm)527
Quantum yield
0.83
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on June 14, 2026