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iFluor® 514 Anti-human CD3 Antibody
UCHT1
The UCHT1 monoclonal antibody binds to human CD3e, a 20 kD member of the Ig superfamily commonly found on the surface of tregs, thymocytes, nkt cells and thymocytes (differentiation dependent)s. In many organisms, CD3 enhances interleukin-4 production, is an inhibitor of gene expression and negatively regulates smoothened signaling pathway. Also, it plays a role in essential cellular pathways, for instance, the cell surface receptor signaling pathway, T cell receptor signaling pathway and apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands like TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 514 Anti-human CD3 Antibody *UCHT1*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B3-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with iFluor® 514 Anti-human CD3 Antibody *UCHT1*. The fluorescence signal was monitored using an Cytek Aurora flow cytometer in the specific B3-A channel.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
10032060100 tests
Price
10032061500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT3E
CloneUCHT1
HostMouse
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Absorbance (nm)511
Correction factor (260 nm)0.265
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)511
Emission (nm)527
Quantum yield
0.83
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Page updated on April 2, 2026