Name: iFluor® 514 Anti-human CD40 Antibody *HI40a*
Brand: AAT Bioquest
SKU: 10400060
Availability: InStock

iFluor® 514 Anti-human CD40 Antibody

HI40a

HI40a is an anti-human monoclonal antibody that targets the CD40 antigen. CD40 (alternatively called TNFRSF5) is a 48 kD transmembrane protein that is found on the surface of cells such as dendritic cells and epithelial cells. CD40 is a member of important cellular pathways, for instance, the tumor necrosis factor-mediated signaling pathway, immune response-regulating cell surface receptor signaling pathway and CD40 signaling pathway. Also, in many organisms, it upregulates GTPase activity, is a promoter of protein kinase C signaling and promotes transcription by RNA polymerase II. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like TRAP and CD154. CD40 is a very popular antibody target, with over 30000 publications in the last decade. CD40 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of cell biology and neuroscience. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
10400060	100 tests	Price
10400061	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD40 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	BP50, TNFRSF5
Clone	HI40a
Host	Mouse
Isotype	IgG2b
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on May 13, 2026