logo
AAT Bioquest

iFluor® 514 Anti-human CD5 Antibody *UCHT2*

Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Spectral properties
Absorbance (nm)511
Correction Factor (260 nm)0.265
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)511
Emission (nm)527
Quantum yield0.831
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
Leu1; Ly-1; T1; Tp67
Clone
UCHT2
Host
mouse
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Absorbance (nm)
511
Correction Factor (260 nm)
0.265
Correction Factor (280 nm)
0.116
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
511
Emission (nm)
527
Quantum yield
0.831
UCHT2 is an anti-human monoclonal antibody that is specific for the CD5 antigen. CD5 (sometimes referred to as Tp67) is a 67 kD transmembrane protein that is found on the surface of cells like T cells and B cells. CD5 is involved with essential cellular pathways, for instance, the apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as CD72, gp35-37, ZAP-70 and TCR. CD5 is a moderately popular antibody target, with over 11000 publications in the last decade. CD5 has been widely used in immunology and costimulatory molecules research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)511
Correction Factor (260 nm)0.265
Correction Factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)511
Emission (nm)527
Quantum yield0.831

Antibody Finder


Select Your CD Antibody
Select Your Clone
Select Your Conjugate

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd5 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.