Name: iFluor® 514 Anti-human CD56 Antibody *B-A19*
Brand: AAT Bioquest
SKU: 10560060
Availability: InStock

iFluor® 514 Anti-human CD56 Antibody

B-A19

B-A19 is an anti-human monoclonal antibody that targets the CD56 antigen. CD56 (alternatively called NKH1 or NCAM-1) is a single-pass type i membrane protein that is expressed on the surface of cells like NK cells and T cells. CD56 has been associated with important biological processes like axon guidance, especially commissural neuron axon guidance. In addition, it plays a role in essential cellular pathways, for instance, the regulation of semaphorin-plexin signaling pathway and interferon-gamma-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as Heparin sulfate. CD56 is a fairly uncommon antibody target, with a little more than 10000 publications in the last decade. Even still, CD56 has a variety of applications in research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10560060	100 tests	Price
10560061	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd56 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Leu-19; NKH1; NCAM1
Clone	B-A19
Host	mouse
Isotype	mouse IgG1
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025