iFluor® 514 Anti-human CD57 Antibody *AHC0061*

iFluor® 514 Anti-human CD57 Antibody

AHC0061

AHC0061 is an anti-human monoclonal antibody that is specific for the CD57 antigen. CD57 (sometimes referred to as LEU7, NK-1 or HNK-1) is a 100 - 115 kD glycoprotein that is expressed on the surface of cells such as T cells and NK cells. CD57 is associated with a variety of biologically interesting macromolecules/ligands, for example, laminin, L-Selectin and P-Selectin. CD57 is a fairly uncommon antibody target, with a little more than 3800 publications in the last decade. Even still, CD57 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to iFluor® 514 (ex/em = 510/527 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the Luminex Guava easyCyte).

Protocol

COA

Catalog	Size	Price	Quantity
10571060	100 tests	Price
10571061	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd57 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	HNK-1, Leu-7
Clone	AHC0061
Host	Mouse
Isotype	Igm, kappa light chain
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Absorbance (nm)	511
Correction factor (260 nm)	0.265
Correction factor (280 nm)	0.116
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	511
Emission (nm)	527
Quantum yield	0.83 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 5, 2024