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iFluor® 514 goat anti-mouse IgG (H+L)
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 514 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~510 nm and ~527 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 514 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 514 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>
<p>HeLa cells were stained with mouse anti-tubulin followed with iFluor<sup>TM</sup> 514 goat anti-mouse IgG (H+L). Cell nuclei were stained with DAPI (Cat#17507).</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16452200 ug
Price
167361 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Absorbance (nm)511
Correction factor (260 nm)0.265
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)511
Emission (nm)527
Quantum yield
0.83
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025