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iFluor® 514 goat anti-rabbit IgG (H+L)
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 514 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~518 nm and ~542 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 514 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).
<p>HeLa cells were incubated with rabbit&nbsp;anti-tubulin followed by iFluor® 514 goat anti-rabbit IgG conjugate. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).</p>
<p>HeLa cells were incubated with rabbit&nbsp;anti-tubulin followed by iFluor® 514 goat anti-rabbit IgG conjugate. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
16612200 ug
Price
168011 mg
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Absorbance (nm)511
Correction factor (260 nm)0.265
Correction factor (280 nm)0.116
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)511
Emission (nm)527
Quantum yield
0.83
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025