iFluor® 532 Anti-human CD107 Antibody *H4B4*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 536 |
Correction Factor (260 nm) | 0.26 |
Correction Factor (280 nm) | 0.16 |
Extinction coefficient (cm -1 M -1) | 900001 |
Excitation (nm) | 537 |
Emission (nm) | 560 |
Quantum yield | 0.681 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names LAMP2; LAMPb | Clone H4B4 | Host mouse | Isotype Mouse IgG1 kappa | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 536 | Correction Factor (260 nm) 0.26 | Correction Factor (280 nm) 0.16 | Extinction coefficient (cm -1 M -1) 900001 | Excitation (nm) 537 | Emission (nm) 560 | Quantum yield 0.681 |
H4B4 is an anti-human monoclonal antibody that targets the CD107b antigen. CD107b (sometimes called LAMP2 or LAMPb) is a 45 kD transmembrane protein that is found on the surface of cells like granulocytes and endothelial cells. In many organisms, CD107 is a suppressor of protein-containing complex assembly. Additionally, it has been associated with essential biological processes like chaperone-mediated autophagy, particularly protein targeting to lysosome involved in chaperone-mediated autophagy. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands. CD107 is a relatively rare antibody target, with fewer than 800 publications in the last decade. Even still, CD107b is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of protein trafficking and clearance and neuroscience. This antibody was purified through affinity chromatography and conjugated to iFluor® 532 (ex/em = 537/560 nm). It is compatible with the 532 nm laser and 575/36 nm bandpass filter (for example, as in the Thermo Fisher Attune NxT).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 536 |
Correction Factor (260 nm) | 0.26 |
Correction Factor (280 nm) | 0.16 |
Extinction coefficient (cm -1 M -1) | 900001 |
Excitation (nm) | 537 |
Emission (nm) | 560 |
Quantum yield | 0.681 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd107b antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?