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iFluor® 532 Anti-human/monkey CD20 Antibody
2H7
The 2H7 monoclonal antibody binds with human/monkey CD20, a 33 - 37 kD transmembrane glycoprotein commonly expressed on the surface of T cells and B cells. CD20 is a component of essential cellular pathways, in particular, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as Lyn. CD20 is a moderately popular antibody target, with over 20000 publications in the last decade. CD20 is vital to immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 532 (ex/em = 537/560 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the Luminex Guava easyCyte).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with iFluor® 532 Anti-human/monkey CD20 Antibody (2H7) (Cat. #10202070). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B3-A channel, demonstrating clear detection of CD20⁺ cells.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with iFluor® 532 Anti-human/monkey CD20 Antibody (2H7) (Cat. #10202070). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the B3-A channel, demonstrating clear detection of CD20⁺ cells.
datasheet
Datasheet
sds
SDS
COA
CatalogSize
Price
Quantity
10202070100 tests
Price
10202071500 tests
Price
 
Antibody properties
Other namesBp35, B1
Clone2H7
HostMouse
IsotypeIgG2b, kappa
ReactivityHuman, Monkey
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Absorbance (nm)536
Correction factor (260 nm)0.26
Correction factor (280 nm)0.16
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)537
Emission (nm)560
Quantum yield
0.68
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on June 4, 2026