Name: iFluor® 532 goat anti-rabbit IgG (H+L)
Brand: AAT Bioquest
SKU: 16616
Availability: InStock

iFluor® 532 goat anti-rabbit IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 532 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~542 nm and ~558 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 532 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

<p>HeLa cells were incubated with rabbit anti-tubulin followed by iFluor® 532 goat anti-rabbit IgG conjugate. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).</p>

Protocol

Catalog	Size	Price	Quantity
16616	200 ug	Price
16802	1 mg	Price

Citations

View all 4 citations: Citation Explorer

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.

Authors:

Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined

Journal:

Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT

Authors:

Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo

Journal:

Tumor Biology (2016): 1--12

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury

Authors:

Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined

Journal:

Acta Biomaterialia (2016): 247--261

The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering

Authors:

Huang, Chuanjun and Zhao, Longxiang and Gu, Jun and Nie, Dekang and Chen, Yinan and Zuo, Hao and Huan, Wei and Shi, Jinlong and Chen, Jian and Shi, Wei

Journal:

Biomedical Materials (2016): 035004

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding

Authors:

Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.

Journal:

Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers

Authors:

Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.

Journal:

Oncotarget (2011): 728

Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient

Authors:

Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.

Journal:

Pharmacotherapy (2010): 954

Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency

Authors:

Buckova A., undefined

Journal:

Epidemiol Mikrobiol Imunol (2010): 133

Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis

Authors:

Boonrod K, Roth B, Leong Ngar S, Krczal G.

Journal:

Anal Biochem (2010): 124

Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Absorbance (nm)	536
Correction factor (260 nm)	0.26
Correction factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	90000 ¹
Excitation (nm)	537
Emission (nm)	560
Quantum yield	0.68 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 16616)
Safety Data Sheet (Catalog 16802)
Certificate of Analysis
Brochure: Secondary Detection Probes & Kits
Flyer: Flow Cytometry Fluorochromes

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Fax
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Page updated on October 30, 2025