Name: iFluor® 546 Anti-human CD25 Antibody *7G7B6*
Brand: AAT Bioquest
SKU: 10251080
Availability: InStock

iFluor® 546 Anti-human CD25 Antibody

7G7B6

The 7G7B6 monoclonal antibody recognizes human CD25, a 55 kD glycoprotein often located on the surface of oligodendrocytes, T cells, B cells and thymocytes. In certain organisms, CD25 negatively regulates defense response to virus, downregulates inflammatory response and positively regulates activated T cell proliferation. Additionally, it is involved with critical cellular pathways, for instance, the interleukin-2-mediated signaling pathway, cell surface receptor signaling pathway and cytokine-mediated signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like IL-2. CD25 is a very popular antibody target, with over 40000 publications in the last decade. CD25 is vital to immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 546 (ex/em = 541/557 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD FACSymphony™ A5).

Datasheet

COA

Catalog	Size	Price	Quantity
10251080	100 tests	Price
10251081	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD25 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	IL-2Rα, p55, TAC antigen
Clone	7G7B6
Host	Mouse
Isotype	IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 21, 2026