Name: iFluor® 546 Anti-human CD71 Antibody *HI160*
Brand: AAT Bioquest
SKU: 10710080
Availability: InStock

iFluor® 546 Anti-human CD71 Antibody

HI160

HI160 is an anti-human monoclonal antibody that is specific for the CD71 antigen. CD71 (also known as Transferrin receptor protein 1) is a 95 kD member of the Transferrin receptor family that is located on the surface of cells such as endothelial cells and stem cells. In certain organisms, CD71 is a positive regulator of isotype switching, is involved in the positive regulation of T cell proliferation and acts to positively regulate B cell proliferation. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands such as Transferrin. CD71 is a fairly uncommon antibody target, with a little more than 4000 publications in the last decade. Even still, CD71 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 546 (ex/em = 541/557 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10710080	100 tests	Price
10710081	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd71 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	T9; Transferrin receptor; TFRC
Clone	HI160
Host	mouse
Isotype	mouse IgG2b
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 21, 2025