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iFluor® 546 Anti-human/monkey CD154 Antibody
5C8
5C8 is an anti-human/monkey monoclonal antibody that forms an immune complex with the CD154 antigen. CD154 (also known as gp39) is a 32 - 39 kD member of the TNF superfamily that is expressed on the surface of cells such as T cells. CD154 is associated with a variety of biologically interesting macromolecules/ligands, for instance, CD40. CD154 is a fairly uncommon antibody target, with a little more than 4000 publications in the last decade. Even still, CD154 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology and costimulatory molecules. This antibody was purified through affinity chromatography and conjugated to iFluor® 546 (ex/em = 541/557 nm). It is compatible with the 532 nm laser and 575/25 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 11540080 | 100 tests | Price | |
| 11540081 | 500 tests | Price |
Antibody properties
| Other names | CD40L, gp39, TRAP, T-BAM |
| Clone | 5C8 |
| Host | Mouse |
| Isotype | IgG2a |
| Reactivity | Human, Monkey |
| Application | Flow Cytometry (FACS), Fluorescence Imaging |
Spectral properties
| Correction factor (260 nm) | 0.25 |
| Correction factor (280 nm) | 0.15 |
| Extinction coefficient (cm -1 M -1) | 100000 1 |
| Excitation (nm) | 541 |
| Emission (nm) | 557 |
| Quantum yield | 0.67 1 |
Usage and storage
| Intended use | Research Use Only (RUO) |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on August 8, 2026