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iFluor® 546 goat anti-rabbit IgG (H+L)
Cross Adsorbed
iFluor® 546 is a bright orange fluorescent dye. iFluor® 546-labeled anti-IgG conjugates exhibit bright fluorescence signal and good photostability. Used for stable signal generation in imaging and flow cytometry, the fluorescence intensity of iFluor® 546 conjugates is pH-insensitive from pH 4 to pH 11. The iFluor® 546-labeled antibody conjugates can be well excited with either Nd:YAG laser (~532 nm) or Helium-Neon laser (~543 nm). iFluor® 546 family has the spectral properties essentially identical to those of Alexa Fluor® 546. Under the same conditions we tested, iFluor® 546 antibody conjugates are brighter and more photostable than the corresponding Alexa Fluo® 546. These spectral and labeling characteristics make the iFluor® 546 dye family a superior alternative to Alexa Fluor® 546. In addition, iFluor® 546 secondary antibody conjugates give higher signal/background ratios than the corresponding Alexa Fluor® 546-labeled conjugates.
<p>HeLa cells were incubated with mouse anti-tubulin followed with&nbsp;iFluor<sup>TM</sup>&nbsp;546 goat anti-rabbit IgG conjugate (Red). Cell nuclei were stained with DAPI&nbsp;(Blue, Cat#17507).</p>
<p>HeLa cells were incubated with mouse anti-tubulin followed with&nbsp;iFluor<sup>TM</sup>&nbsp;546 goat anti-rabbit IgG conjugate (Red). Cell nuclei were stained with DAPI&nbsp;(Blue, Cat#17507).</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16688200 ug
Price
 
Physical properties
Molecular weight~150000
SolventWater
Spectral properties
Correction factor (260 nm)0.25
Correction factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)541
Emission (nm)557
Quantum yield
0.67
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025