Name: iFluor® 555 Anti-human CD3 Antibody *HIT3b*
Brand: AAT Bioquest
SKU: 10031090
Availability: InStock

iFluor® 555 Anti-human CD3 Antibody

HIT3b

The HIT3b monoclonal antibody binds to human CD3e, a 20 kD single-pass type i membrane protein commonly located on the surface of nkt cells, tregs, thymocytes (differentiation dependent)s, thymocytes and t cells. In many organisms, CD3 positively regulates T cell anergy, is a promoter of peptidyl-tyrosine phosphorylation and enhances interferon-gamma production. Also, it is a component of vital cellular pathways, namely, the G protein-coupled receptor signaling pathway, cell surface receptor signaling pathway and negative regulation of smoothened signaling pathway. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as TCR. CD3 is a very popular antibody target, with over 80000 publications in the last decade. CD3e is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 555 (ex/em = 557/570 nm). It is compatible with the 561 nm laser and 582/15 nm bandpass filter (for example, as in the BD FACSAria™ Fusion).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10031090	100 tests	Price
10031091	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd3e antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	T3E
Clone	HIT3b
Host	mouse
Isotype	mouse IgG1
Reactivity	human
Application	Flow Cytometry (FACS); Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025