Name: iFluor® 555 goat anti-rabbit IgG (H+L)
Brand: AAT Bioquest
SKU: 16620
Availability: InStock

iFluor® 555 goat anti-rabbit IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 555 goat anti-rabbit IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~559 nm and ~569 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 555 goat anti-rabbit IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

<p>HeLa cells were stained with rabbit anti-tubulin followed with iFluor<sup>TM</sup> 555 goat anti-rabbit IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor<sup>TM</sup> 488 conjugate (green); and nuclei were stained with DAPI (blue).</p>

Protocol

Catalog	Size	Price	Quantity
16620	200 ug	Price
16803	1 mg	Price

Citations

View all 7 citations: Citation Explorer

Specific induction of right ventricular-like cardiomyocytes from human pluripotent stem cells

Authors:

Saito, Yukihiro and Nakamura, Kazufumi and Katanosaka, Yuki and Iida, Toshihiro and Kusumoto, Dai and Sato, Ryushi and Adachi, Riki and Shimizu, Satoshi and Kurokawa, Junko and Akagi, Satoshi and others,

Journal:

Stem Cell Research \& Therapy (2025): 519

Dose-Dependent Effect of a New Biotin Compound in Hippocampal Remyelination in Rats

Authors:

Yulug, Burak and Kilic, Ertugrul and O{\u{g}}uz, Tuba and Orhan, Cemal and Er, Besir and Tuzcu, Mehmet and Ozercan, Ibrahim Hanifi and Sahin, Nurhan and Canpolat, Sinan and Komorowski, James and others,

Journal:

Molecular Neurobiology (2025): 1--18

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate

Authors:

Li, Min and Min, Wei and Wang, Jianbo and Wang, Lu and Li, Yan and Zhou, Naihui and Yang, Ziliang and Qian, Qihong

Journal:

Experimental and Therapeutic Medicine (2020): 2861--2870

Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.

Authors:

Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined

Journal:

Oncotarget (2017)

Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT

Authors:

Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo

Journal:

Tumor Biology (2016): 1--12

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding

Authors:

Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.

Journal:

Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers

Authors:

Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.

Journal:

Oncotarget (2011): 728

Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency

Authors:

Buckova A., undefined

Journal:

Epidemiol Mikrobiol Imunol (2010): 133

Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies

Authors:

Azzopardi P, Forrester M, Ehtisham S.

Journal:

J Paediatr Child Health (2010): 439

Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies

Authors:

Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.

Journal:

Anal Chim Acta (2010): 126

Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 16620)
Safety Data Sheet (Catalog 16803)
Certificate of Analysis
Brochure: Secondary Detection Probes & Kits

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Page updated on October 30, 2025