Name: iFluor® 560 Anti-human CD27 Antibody *LT27*
Brand: AAT Bioquest
SKU: 102700A0
Availability: InStock

iFluor® 560 Anti-human CD27 Antibody

LT27

LT27 is an anti-human monoclonal antibody that recognizes the CD27 antigen. CD27 (sometimes referred to as T14, S152 or TNFRSF7) is a 50 - 55 kD member of the TNF-R superfamily that is expressed on the surface of cells such as NK cells, B cells and T cells. In some organisms, CD27 is a promoter of T cell differentiation, acts to positively regulate NIK/NF-kappaB signaling and is involved in the negative regulation of apoptotic process. Furthermore, it plays a role in key cellular pathways, in particular, the tumor necrosis factor-mediated signaling pathway, cell surface receptor signaling pathway and extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like TRAF5, TRAF2 and CD70. CD27 is a moderately popular antibody target, with over 11000 publications in the last decade. CD27 is essential for immunology and costimulatory molecules research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 560 (ex/em = 560/571 nm). It is compatible with the 561 nm laser and 577/15 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).

Datasheet

COA

Catalog	Size	Price	Quantity
102700A0	100 tests	Price
102700A1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD27 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	T14, S152, TNFRSF7
Clone	LT27
Host	Mouse
Isotype	IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.0482
Correction factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.57 ¹

Usage and storage

Intended use

Research Use Only (RUO)

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Custom size	Inquire
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Shipping	Standard overnight for United States, inquire for international

Page updated on August 22, 2026