Name: iFluor® 560 Anti-human CD55 Antibody *HI55a*
Brand: AAT Bioquest
SKU: 105500A0
Availability: InStock

iFluor® 560 Anti-human CD55 Antibody

HI55a

HI55a is an anti-human monoclonal antibody that targets the CD55 antigen. CD55 (sometimes called Complement decay accelerating factor or DAF (Decay Accelerating Factor)) is a 60 - 70 kD single-pass type i membrane protein that is found on the surface of cells like macrophages, platelets, granulocytes, T cells and NK cells. CD55 is a component of vital cellular pathways, for example, the complement activation, classical pathway and regulation of lipopolysaccharide-mediated signaling pathway. Additionally, in some organisms, it is involved in the positive regulation of CD4-positive, alpha-beta T cell activation, upregulates CD4-positive, alpha-beta T cell proliferation and acts to positively regulate cytosolic calcium ion concentration. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like SCR, CD97 and Echoviruses. CD55 is a fairly uncommon antibody target, with a little more than 3000 publications in the last decade. Even still, CD55 is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of cell biology, neuroinflammation and immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 560 (ex/em = 560/571 nm). It is compatible with the 561 nm laser and 582/15 nm bandpass filter (for example, as in the BD FACSAria™ III).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
105500A0	100 tests	Price
105500A1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd55 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	DAF
Clone	HI55a
Host	Mouse
Isotype	Mouse IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.0482
Correction factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000 ¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.57 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 21, 2025