iFluor® 568 Anti-human CD120a Antibody *H398*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.34 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 568 |
Emission (nm) | 587 |
Quantum yield | 0.571 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names TNFR-I; TNFRSF1A; P60; P55 | Clone H398 | Host mouse | Isotype Mouse IgG2a | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.34 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 1000001 | Excitation (nm) 568 | Emission (nm) 587 | Quantum yield 0.571 |
H398 is an anti-human monoclonal antibody that is specific for the CD120a antigen. CD120a (alternatively called P55, TNFR-I, P60 or TNFRSF1A) is a 55 kD member of the TNFR superfamily that is expressed on the surface of cells such as B cells and T cells. CD120a is associated with a variety of biologically interesting macromolecules/ligands, for instance, TRAF, TRADD and ltα. CD120a is a relatively rare antibody target, with fewer than 300 publications in the last decade. Even still, CD120a is typically used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 568 (ex/em = 568/587 nm). It is compatible with the 561 nm laser and 577/15 nm bandpass filter (for example, as in the Bio-Rad ZE5 Cell Analyzer).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.34 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 568 |
Emission (nm) | 587 |
Quantum yield | 0.571 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd120a antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?