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iFluor® 568 Anti-human CD21 Antibody *HI21a*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
CR2; C3dR; Epstein-Barr virus receptor
Clone
HI21a
Host
mouse
Isotype
mouse IgG2a
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.34
Correction Factor (280 nm)
0.15
Extinction coefficient (cm -1 M -1)
1000001
Excitation (nm)
568
Emission (nm)
587
Quantum yield
0.571
The HI21a monoclonal antibody recognizes human CD21, a 145 kD transmembrane glycoprotein frequently expressed on the surface of thymocytes and dendritic cells. CD21 plays a role in vital cellular pathways, namely, the complement activation, classical pathway. In addition, it has been associated with vital biological processes like immune response, specifically innate immune response. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands such as CD19, CD23 and C3d. CD21 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD21 is essential for complement, innate immunity and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 568 (ex/em = 568/587 nm). It is compatible with the 561 nm laser and 583/24 nm bandpass filter (for example, as in the Luminex Amnis CellStream).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd21 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.