iFluor® 594 Anti-human CD8 Antibody *OKT-8*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 587 |
Emission (nm) | 603 |
Quantum yield | 0.531 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names T8; Leu2 | Clone OKT-8 | Host mouse | Isotype mouse igg2a | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Absorbance (nm) 587 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 2000001 | Excitation (nm) 587 | Emission (nm) 603 | Quantum yield 0.531 |
OKT-8 is an anti-human monoclonal antibody that forms an immune complex with the CD8a antigen. CD8a (alternatively called T8 or MAL) is a 32 - 34 kD transmembrane glycoprotein that is located on the surface of cells such as T cells. CD8 is a component of essential cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 120000 publications in the last decade. CD8a has a variety of applications in immunology research, commonly serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 594 (ex/em = 588/604 nm). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 2000001 |
Excitation (nm) | 587 |
Emission (nm) | 603 |
Quantum yield | 0.531 |
Antibody Finder
Select Your CD Antibody | |
Select Your Clone | |
Select Your Conjugate |
Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd8a antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Citations
View all 1 citations: Citation Explorer
Anti-SARS-CoV-2 T-stem cell memory persists in ocrelizumab-treated MS patients
Authors: Guerrera, Gisella and Mandelli, Alessandra and Finardi, Annamaria and Orrico, Mario and D’Orso, Silvia and Picozza, Mario and Noviello, Maddalena and Beretta, Valeria and Bonetti, Bruno and Calabrese, Massimiliano and others,
Journal: Multiple Sclerosis Journal (2022): 1937--1943
Authors: Guerrera, Gisella and Mandelli, Alessandra and Finardi, Annamaria and Orrico, Mario and D’Orso, Silvia and Picozza, Mario and Noviello, Maddalena and Beretta, Valeria and Bonetti, Bruno and Calabrese, Massimiliano and others,
Journal: Multiple Sclerosis Journal (2022): 1937--1943
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?