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AAT Bioquest

iFluor® 594 Goat Anti-human IgG (H+L) Antibody

AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This iFluor® 594-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar iFluor® 594 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to iFluor® 594 (ex/em = 588/604 nm). It is compatible with the 561 nm laser and 585/20 nm bandpass filter (for example, as in the BD Special Order LSRFortessa™ Cell Analyzer).

Spectrum

Conjugation

We provide custom conjugation services for this antibody (eg. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.
Page updated on October 12, 2024

Ordering information

Price
Unit size
200 ug
1 mg
Catalog Number
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Antibody properties

Other names
Hostgoat
Reactivityhuman
ApplicationFlow Cytometry (FACS); ELISA; IHC; Western Blot

Spectral properties

Absorbance (nm)

587

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.04

Extinction coefficient (cm -1 M -1)

2000001

Excitation (nm)

587

Emission (nm)

603

Quantum yield

0.531

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22