iFluor® 594 goat anti-mouse IgG (H+L)

Name: iFluor® 594 goat anti-mouse IgG (H+L)
Brand: AAT Bioquest
SKU: 16468
Availability: InStock

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 594 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~592 nm and ~614 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 594 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 350 goat anti-mouse IgG (H+L) Cross Adsorbed	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-mouse IgG (H+L)	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 405 goat anti-mouse IgG (H+L) Cross Adsorbed	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 goat anti-mouse IgG (H+L)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 488 goat anti-mouse IgG (H+L) Cross Adsorbed	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 514 goat anti-mouse IgG (H+L)	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 514 goat anti-mouse IgG (H+L) Cross Adsorbed	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-mouse IgG (H+L)	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 532 goat anti-mouse IgG (H+L) Cross Adsorbed	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 546 goat anti-mouse IgG (H+L)	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 546 goat anti-mouse IgG (H+L) Cross Adsorbed	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 555 goat anti-mouse IgG (H+L)	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 555 goat anti-mouse IgG (H+L) Cross Adsorbed	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 568 goat anti-mouse IgG (H+L)	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 568 goat anti-mouse IgG (H+L) Cross Adsorbed	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 594 goat anti-rabbit IgG (H+L)	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 594 goat anti-rabbit IgG (H+L) Cross Adsorbed	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-mouse IgG (H+L)	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 633 goat anti-mouse IgG (H+L) Cross Adsorbed	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-mouse IgG (H+L)	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 647 goat anti-mouse IgG (H+L) Cross Adsorbed	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-mouse IgG (H+L)	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 680 goat anti-mouse IgG (H+L) Cross Adsorbed	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-mouse IgG (H+L)	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 700 goat anti-mouse IgG (H+L) Cross Adsorbed	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-mouse IgG (H+L)	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 750 goat anti-mouse IgG (H+L) Cross Adsorbed	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-mouse IgG (H+L)	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 790 goat anti-mouse IgG (H+L) Cross Adsorbed	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 goat anti-mouse IgG (H+L)	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 800 goat anti-mouse IgG (H+L) Cross Adsorbed	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 goat anti-mouse IgG (H+L)	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 810 goat anti-mouse IgG (H+L) Cross Adsorbed	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 goat anti-mouse IgG (H+L)	822	850	250000¹		0.11	0.16
iFluor® 820 goat anti-mouse IgG (H+L) Cross Adsorbed	822	850	250000¹		0.11	0.16
iFluor® 840 goat anti-mouse IgG (H+L)	836	879	200000¹	-	0.2	0.09
iFluor® 840 goat anti-mouse IgG (H+L) Cross Adsorbed	836	879	200000¹	-	0.2	0.09
iFluor® 860 goat anti-mouse IgG (H+L)	853	878	250000¹		0.1	0.14
iFluor® 860 goat anti-mouse IgG (H+L) Cross Adsorbed	853	878	250000¹		0.1	0.14
Show More (31)

Citations

View all 14 citations: Citation Explorer

Aspirin reverses the inhibitory effect of soluble fms-like tyrosine kinase-1 on trophoblast invasiveness and ciliogenesis through Sonic hedgehog signaling in preeclampsia
Authors: Chen, Yu-Jen and Lee, Chih-I and Tsai, Pei-Yin and Tam, Hoi-Lam and Su, Mei-Tsz
Journal: Biochemical Pharmacology (2025): 116975

TiO2 Nanotube Implants Modified with Silk Fibroin and Mesoporous Silica Nanocomposite Coatings Enable Efficient Drug Release to Promote Osteogenesis
Authors: Mu, Yanting and Li, Ming and Zhao, Xiang and Gong, Chaihong and Luo, Zhang and Li, Bing and Zhang, Weiying and Ge, Xiaoxiao and Chen, Su and Zhou, Jian
Journal: ACS Applied Materials \& Interfaces (2025)

USP20 mitigates doxorubicin-induced cardiotoxicity by deubiquitinating and stabilizing HuR
Authors: Huang, Zhouqing and Chen, Yunxuan and Wu, Shuoning and Deng, Lang and Zhou, Yixin and Zhang, Yucong and Mei, Jiaxuan and Wang, Fang and Shen, Sirui and Fang, Ziming and others,
Journal: (2025)

SLC7A5 regulates tryptophan uptake and PD-L1 expression levels via the kynurenine pathway in ovarian cancer
Authors: Jiang, Ruibin and Jin, Bo and Sun, Yuting and Chen, Zhongjian and Wan, Danying and Feng, Jianguo and Ying, Lisha and Peng, Chanjuan and Gu, Linhui
Journal: Oncology Letters (2025): 1--15

Natural lung-tropic TH9 cells: a sharp weapon for established lung metastases
Authors: Chen, Tao and Qiao, Chenxiao and Yinwang, Eloy and Wang, Shengdong and Wen, Xuehuan and Feng, Yixuan and Jin, Xiangang and Li, Shuming and Xue, Yucheng and Zhou, Hao and others,
Journal: Journal for ImmunoTherapy of Cancer (2024): e009629

References

View all 191 references: Citation Explorer

Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668

Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728

Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954

Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133

Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124

Page updated on June 10, 2025

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Unit size	200 ug 1 mg
Catalog Number	1646816741
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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Absorbance (nm)	587
Correction Factor (260 nm)	0.05
Correction Factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501