iFluor® 610 Anti-human CD44 Antibody *HI44a*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.49 |
Extinction coefficient (cm -1 M -1) | 1100001 |
Excitation (nm) | 610 |
Emission (nm) | 628 |
Quantum yield | 0.851 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names ECMR-III; Pgp-1; HUTCH-1; H-CAM | Clone HI44a | Host mouse | Isotype mouse IgG2a | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.49 | Extinction coefficient (cm -1 M -1) 1100001 | Excitation (nm) 610 | Emission (nm) 628 | Quantum yield 0.851 |
The HI44a monoclonal antibody reacts with human CD44, a 85 kD transmembrane glycoprotein commonly expressed on the surface of leukocytes, endothelial cells, lymphohematopoietic cells, epithelial cells and hepatocytes. In many organisms, CD44 acts to positively regulate peptidyl-tyrosine phosphorylation, enhances peptidyl-serine phosphorylation and is a positive regulator of ERK1 and ERK2 cascade. Also, it acts in critical cellular pathways, namely, the interferon-gamma-mediated signaling pathway and negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands such as Matrix metalloprotein- ases (MMPs), Collagen, Osteopontin and Hyaluronan. CD44 is a very popular antibody target, with over 40000 publications in the last decade. CD44 has been widely used in immunology research, typically serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 610 (ex/em = 610/628 nm). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).
Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.49 |
Extinction coefficient (cm -1 M -1) | 1100001 |
Excitation (nm) | 610 |
Emission (nm) | 628 |
Quantum yield | 0.851 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd44 antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?