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iFluor® 610 Anti-human CD72 Antibody *3F3*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.49
Extinction coefficient (cm -1 M -1)1100001
Excitation (nm)610
Emission (nm)628
Quantum yield0.851
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
 Ly-19.2;  Ly-32.2; Lyb-2
Clone
3F3
Host
mouse
Isotype
Mouse IgG2b
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.49
Extinction coefficient (cm -1 M -1)
1100001
Excitation (nm)
610
Emission (nm)
628
Quantum yield
0.851
The 3F3 monoclonal antibody binds with human CD72, a 39 - 43 kD transmembrane glycoprotein often located on the surface of B cells, dendritic cells, liver kupffer cells and macrophages. CD72 is associated with a variety of biologically interesting macromolecules/ligands, for example, CD5. CD72 is a relatively rare antibody target, with fewer than 500 publications in the last decade. Even still, CD72 is commonly used in flow cytometry applications as a phenotypic marker for differentiation of cell types, especially in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 610 (ex/em = 610/628 nm). It is compatible with the 592 nm laser and 610/30 nm bandpass filter (for example, as in the Luminex Amnis ImageStream).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.49
Extinction coefficient (cm -1 M -1)1100001
Excitation (nm)610
Emission (nm)628
Quantum yield0.851

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd72 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.