iFluor® 633 Anti-human CD11b Antibody *HI11b*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | water |
Spectral properties
Correction Factor (260 nm) | 0.062 |
Correction Factor (280 nm) | 0.044 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 640 |
Emission (nm) | 654 |
Quantum yield | 0.291 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
Overview | SDSProtocol |
Other names CR3; Mac-1; Mo1; ITGAM; Integrin alpha-M | Clone HI11b | Host mouse | Isotype mouse IgG2b | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Molecular weight N/A | Correction Factor (260 nm) 0.062 | Correction Factor (280 nm) 0.044 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 640 | Emission (nm) 654 | Quantum yield 0.291 |
HI11b is an anti-human monoclonal antibody that recognizes the CD11b antigen. CD11b (sometimes referred to as Integrin αM chain, C3biR, CR3A or CR3) is a 165 - 170 kD glycoprotein that is expressed on the surface of cells such as B cells, granulocytes, NK cells, macrophages and dendritic cells. CD11b is involved with important cellular pathways, in particular, the cytokine-mediated signaling pathway, toll-like receptor 4 signaling pathway and apoptotic signaling pathway. Additionally, in many organisms, it is a promoter of superoxide anion generation, positively regulates microglial cell mediated cytotoxicity and is a positive regulator of prostaglandin-E synthase activity. CD11b has been associated with critical biological processes like cell adhesion, specifically cell-cell adhesion via plasma-membrane adhesion molecules, and is associated with a variety of biologically interesting macromolecules/ligands, for example, Factor X, iC3b and ICAM-1. CD11b is a very popular antibody target, with over 45000 publications in the last decade. CD11b has been widely used in innate immunity, neuroscience cell markers and cell biology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 633 (ex/em = 640/654 nm). It is compatible with the 638 nm laser and 660/20 nm bandpass filter (for example, as in the Beckman Coulter Navios EX).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.062 |
Correction Factor (280 nm) | 0.044 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 640 |
Emission (nm) | 654 |
Quantum yield | 0.291 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd11b antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?