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iFluor® 633 Anti-human CD43 Antibody *HI161*

Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Spectral properties
Correction Factor (260 nm)0.062
Correction Factor (280 nm)0.044
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)640
Emission (nm)654
Quantum yield0.291
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Overview


Other names
Leukocyte Sialoglycoprotein; Leukosialin; Galactoglycoprotein; SPN
Clone
HI161
Host
mouse
Isotype
mouse IgG1
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.062
Correction Factor (280 nm)
0.044
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
640
Emission (nm)
654
Quantum yield
0.291
The HI161 monoclonal antibody reacts with human CD43, a 95 - 135 kD transmembrane protein commonly located on the surface of plasma cells, thymocytes, neutrophils, myelomas and T cells. In some organisms, CD43 plays a role in the downregulation of cell adhesion, promotes tumor necrosis factor biosynthetic process and acts to negatively regulate T cell proliferation. From a research standpoint, it is of biological interest due to its association with vital macromolecules/ligands such as EZR. CD43 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD43 has been widely used in immunology research, frequently serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 633 (ex/em = 640/654 nm). It is compatible with the 640 nm laser and 667/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.062
Correction Factor (280 nm)0.044
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)640
Emission (nm)654
Quantum yield0.291

Antibody Finder


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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd43 antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.