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AAT Bioquest

iFluor® 633 Anti-human CD45 Antibody *UCHL1*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Spectral properties
Correction Factor (260 nm)0.062
Correction Factor (280 nm)0.044
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)640
Emission (nm)654
Quantum yield0.291
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

OverviewpdfSDSpdfProtocol


Other names
CD45RO
Clone
UCHL1
Host
mouse
Isotype
Mouse IgG2a, κ
Reactivity
human
Application
Flow Cytometry (FACS); Fluorescence Imaging
Correction Factor (260 nm)
0.062
Correction Factor (280 nm)
0.044
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
640
Emission (nm)
654
Quantum yield
0.291
UCHL1 is an anti-human monoclonal antibody that forms an immune complex with the CD45ro antigen. CD45ro (sometimes called CD45RO) is a 180 kD transmembrane glycoprotein that is found on the surface of cells like granulocytes, macrophages and NK cells. In certain organisms, CD45 plays a role in the upregulation of protein kinase activity, is a positive regulator of tumor necrosis factor production and is a negative regulator of protein kinase activity. Additionally, it has been associated with key biological processes like dephosphorylation, particularly protein dephosphorylation. CD45 acts in key cellular pathways, for example, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Src kinases, p56lck and p59fyn. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ro is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 633 (ex/em = 640/654 nm). It is compatible with the 633 nm laser and 660/20 nm bandpass filter (for example, as in the BD FACSAria™ III).

Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.062
Correction Factor (280 nm)0.044
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)640
Emission (nm)654
Quantum yield0.291

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Conjugation


We provide custom conjugation services for this antibody (eg. labeling of cd45ro antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.