Name: iFluor® 633 Anti-human CD47 Antibody *HI172*
Brand: AAT Bioquest
SKU: 104710E0
Availability: InStock

iFluor® 633 Anti-human CD47 Antibody

HI172

HI172 is an anti-human monoclonal antibody that is specific for the CD47 antigen. CD47 (sometimes called Integrin associated protein) is a 42 - 52 kD multi-pass membrane protein that is found on the surface of cells such as granulocytes, platelets, endothelial cells and B cells. CD47 acts in critical cellular pathways, in particular, the negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis and integrin-mediated signaling pathway. In addition, in certain organisms, it downregulates Fc-gamma receptor signaling pathway involved in phagocytosis, upregulates T cell activation and acts to positively regulate inflammatory response. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like SIRP, Thrombospondin and CD61. CD47 is a fairly uncommon antibody target, with a little more than 5000 publications in the last decade. Even still, CD47 is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to iFluor® 633 (ex/em = 640/654 nm). It is compatible with the 640 nm laser and 675/25 nm bandpass filter (for example, as in the BD Accuri™ C6 Plus).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
104710E0	100 tests	Price
104710E1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of cd47 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	gp42, IAP, neurophilin, MER6, Integrin associated protein
Clone	HI172
Host	Mouse
Isotype	Mouse IgG1
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.062
Correction factor (280 nm)	0.044
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	640
Emission (nm)	654
Quantum yield	0.29 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 19, 2025