iFluor® 647 Anti-human CD20 Antibody *HI47*

iFluor® 647 Anti-human CD20 Antibody

HI47

HI47 is an anti-human monoclonal antibody that recognizes the CD20 antigen. CD20 (alternatively called Leukocyte surface antigen Leu-16) is a 33 - 37 kD transmembrane protein that is located on the surface of cells such as B cells and T cells. CD20 is a member of critical cellular pathways, in particular, the B cell receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with essential macromolecules/ligands like lyn. CD20 is a very popular antibody target, with over 28000 publications in the last decade. CD20 has been widely used in immunology and costimulatory molecules research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 647 (ex/em = 656/670 nm). It is compatible with the 638 nm laser and 660/20 nm bandpass filter (for example, as in the Beckman Coulter Navios EX).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
102000F0	100 tests	Price
102000F1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD20 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	Bp35, B1
Clone	HI47
Host	Mouse
Isotype	IgG3
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 5, 2024