Name: iFluor® 647 Anti-human CD54 Antibody *R6-5-D6*
Brand: AAT Bioquest
SKU: 105400F0
Availability: InStock

iFluor® 647 Anti-human CD54 Antibody

R6-5-D6

R6-5-D6 is an anti-human monoclonal antibody that targets the CD54 antigen. CD54 (also known as ICAM-1, ICAM1 or Ly-47) is a 85 - 110 kD member of the Ig superfamily that is expressed on the surface of cells such as B cells, endothelial cells and macrophages. CD54 is associated with a variety of biologically interesting macromolecules/ligands, for example, LFA-1, Mac-1 and Rhinovirus. CD54 is a fairly uncommon antibody target, with a little more than 4400 publications in the last decade. Even still, CD54 is essential for costimulatory molecules, neuroscience and immunology research, often serving as a phenotypic marker for differentiating cell types in flow cytometric applications. This antibody was purified through affinity chromatography and conjugated to iFluor® 647 (ex/em = 656/670 nm). It is compatible with the 640 nm laser and 667/30 nm bandpass filter (for example, as in the Agilent Technologies NovoCyte Advanteon).

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
105400F0	100 tests	Price
105400F1	500 tests	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of CD54 antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Antibody properties

Other names	ICAM-1
Clone	R6-5-D6
Host	Mouse
Isotype	IgG2a
Reactivity	Human
Application	Flow Cytometry (FACS), Fluorescence Imaging

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on June 19, 2026